JC-1 is , while it's Molecular Formula is C25H27Cl4IN4. The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.

What is the CAS number for JC-1?

The CAS number of JC-1 is 3520-43-2.

What is JC-1 (3520-43-2) used for?

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry. A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers). JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.InChI:InChI=1/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

JC-1 is , while it's Molecular Formula is C25H27Cl4IN4. The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.

What is the CAS number for JC-1?

The CAS number of JC-1 is 3520-43-2.

What is JC-1 (3520-43-2) used for?

JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis. It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry. A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers). JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.InChI:InChI=1/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

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3520-43-2

Product Name:JC-1
Molecular Formula:C₂₅H₂₇Cl₄IN₄
Molecular Weight:652.22

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Product Details

pd_meltingpoint:275–278 ℃

Purity:99%

Factory supply good quality JC-1 3520-43-2 with stock

Molecular Formula:C25H27Cl4IN4
Molecular Weight:652.22
Melting Point:275–278 ℃
PSA：18.67000
LogP:4.15720

JC-1(Cas 3520-43-2) Usage

InChI:InChI=1/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

3520-43-2 Relevant articles

MACROPHAGE IDENTIFICATION AGENT, AND IDENTIFICATION METHOD, SORTING METHOD, EVALUATION METHOD, SCREENING METHOD AND KIT USING THE MACROPHAGE IDENTIFIER AGENT

-

Paragraph 0363, (2016/02/28)

An object of the present invention is to...

The structure of benzimidazole cyanine dyes, their spectroscopy and their performance in photographic emulsions

Peng, Zheng-Hong,Geise,Zhou, Xiang-Feng,Peng, Bi-Xian,Carleer,Dommisse

, p. 27 - 33 (2007/10/03)

The multi-step synthesis of two benzimid...

3520-43-2 Process route

: 4751-25-1
2-(2'-anilinoethenyl)-5,6-dichloro-1,3-diethylbenzimidazole

: 3520-43-2,47729-63-5,440642-60-4
5,6-dichloro-2-<1-<3-(5,6-dichloro-1,3-diethyl-2-benzimidazolylidene)propenyl>>-1,3-diethylbenzimidazolium iodide

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: 72 percent / acetone / 4 h / Ambient temperature 2: 1.) NEt₃, pyridine, 2.) 25percent aq. KI / 1.) reflux, 2.) reflux With pyridine; triethylamine; potassium iodide; In acetone;

: 3237-62-5
5,6-dichloro-1-ethyl-2-methyl benzimidazole

: 3520-43-2,47729-63-5,440642-60-4
5,6-dichloro-2-<1-<3-(5,6-dichloro-1,3-diethyl-2-benzimidazolylidene)propenyl>>-1,3-diethylbenzimidazolium iodide

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: 100 percent / 2 h / 160 °C 2: 1.) NEt₃, pyridine, 2.) 25percent aq. KI / 1.) reflux, 2.) reflux With pyridine; triethylamine; potassium iodide;

3520-43-2 Upstream products

75-03-6
ethyl iodide
3237-62-5
5,6-dichloro-1-ethyl-2-methyl benzimidazole
80-40-0
ethyl ester of p-toluenesulfonic acid
4751-25-1
2-(2'-anilinoethenyl)-5,6-dichloro-1,3-diethylbenzimidazole

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